| Biosafety Level 2 |
Yes |
No |
N/A |
Comments |
| A. Standard Microbiological Practices |
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| 1. Access limited at when experiments in progress (discretionary) |
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| 2. Persons wash hands after work w/ cultures & removing gloves, before leaving lab |
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| 3. Eating, drinking, storing food, etc. prohibited |
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| 4.. Mouth pipetting prohibited; pipettors used |
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| 5. Sharps policies in place |
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| 6. Splashes & aerosols are minimized |
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| 7. Work surfaces disinfected 1x per day and after spills, disinfectants effective |
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| 8. Regulated waste disposed properly. |
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| 9. Insect & rodent control program in place. |
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| B. Special Practices: |
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| 1. Lab access restricted when working with infectious agents. |
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| 2. Policies so that persons advised of hazards & have required immunizations. |
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| 3. Biohazard sign must be present: agent, BSL, PPE, exit require., name, phone. |
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| 4. Lab personnel receive appropriate immunizations & tests for agents handled. |
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| 5. Baseline serum collected, as appropriate. |
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| 6. Biosafety manual adopted. Persons informed of special hazards. |
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| 7. Director ensures personnel receive appropriate training & annual updates. |
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| 8. Sharps precautions: needles, slides, pipettes, cap. tubes, scalpels: |
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| a. Sharps restricted to use when no alternative exists. |
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| b. Needles are integral to syringe and not recapped, bent, etc. |
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| c. Safe needle devices used where appropriate. |
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| d. Broken glassware handled by mechanical means. |
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| 9. Specimen containers leakproof and covered during transport. |
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| 10. Equipment & work surfaces disinfected regularly, after work w/ agents, after spills. |
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| 11. Spills & accidents reported to lab director. Medical follow-up as appropriate. |
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| 12. Animals not involved in work not permitted in lab. |
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| C. Safety Equipment (Primary Barriers) |
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| 1. Biosafety cabinet (Class II) and other containment devices or PPE used when: |
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| a. Potential for aerosols or splashes exist. |
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| b. High concentrations or large volumes of agents are used. |
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| 2. Face protection used for work outside BSC that may generate splashes. |
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| 3. Lab coats worn and removed prior to leaving lab. Laundered by the institution. |
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| 4. Gloves worn when working with agents. Alternatives to powered latex available. |
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| D. Laboratory Facilities (Secondary Barriers) |
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| 1. Provide lockable doors for restricted agents. (42 CFR 72.6) |
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| 2. Locate new labs away from public areas. |
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| 3. Labs have handwash sink |
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| 4. Easily cleaned. No carpets or rugs |
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| 5. Benchtops impervious to water and resistant to chemicals |
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| 6. Lab furniture is appropriate for loading and use. Spaces accessible for cleaning |
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| 7. BSC’s located away from doors, heavily traveled areas, etc, to maintain air flow. |
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| 8. Eyewash readily available. |
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| 9. Illumination is adequate, avoiding glares and reflections |
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| 10. Negative airflow recommended. Windows have flyscreens. |
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| 11. Are autoclaving procedures verified? Yes No If yes, explain how?
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| E. Training of Personnel |
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| Documented lab safety training? |
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| Documented bloodborne pathogens/biosafety training? |
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