Office: MSB, G665
Lab:


Email: parky1@umdnj.edu

Roles of non-coding RNAs in gene regulation and chromatin organization

To understand several aspects of gene regulation in eukaryotes which is achieved by histone modification and chromatin remodeling, our research is focused on understanding the relationship between chromatin organization and non-coding RNAs by studying a model of dosage compensation of the male X chromosome in Drosophila melanogaster. MSL (Male Specific Lethal) complex binds to a male X chromosome and induces 2-fold hypertranscription to equalize the amounts of gene product from one male X chromosome with two female X chromosomes. The MSL complex is composed of at least 6 proteins including two histone modifying enzymes and two non-coding roX (RNA on X) RNAs. Interestingly, the two non-coding roX RNAs, roX1 and roX2, are not only components of the MSL complex, but also are two major MSL spreading initiation sites on the X chromosome. Even though roX1 (3.7 kb) and roX2 (0.5 kb) are very different in size and sequence, they have redundant functions. However, the exact role of these RNAs in targeting and spreading of MSL complex to the male X chromosome is unknown. Therefore, characterization of the function of these roX RNAs will be a key understanding how a RNP (Ribonucleoprotein) complex regulates chromatin organization.

We are currently 1) characterizing functional significance of the alternative splicing of roX RNAs in MSL complex, 2) defining functional domains of roX RNAs and interactions with MSL proteins, 3) analyzing molecular basis of MSL cis-spreading from roX genes, and 4) screening new non-coding RNA(s) interacting with MLE (RNA helicase).

Representative Publications:

• Meller VH, Gordadze PR, Park Y, Chu X, Stuckenholz C, Kelley RL, and Kuroda MI. (2000) Ordered assembly of roX RNAs into MSL complexes on the dosage-compensated X chromosome in Drosophila, Curr. Biol. 10(3), 136-143.

• Park Y and Kuroda MI. (2001) Epigenetic aspects of X-chromosome dosage compensation, Science, 293(5532), 1083-1085.

• Park Y, Kelley RL, Oh H, Kuroda MI, and Meller VH. (2002) Extent of chromatin spreading determined by roX RNA Recruitment of MSL proteins, Science, 298(5598), 1620-1623.

• Park Y, Mengus G, Bai X, Kageyama Y, Meller VH, Becker PB, and Kuroda MI. (2003) Sequence-specific targeting of Drosophila roX genes by the MSL dosage compensation complex, Mol. Cell, 11(4), 977-986.

• Oh H, Park Y, and Kuroda MI. (2003) Local spreading of MSL complexes from roX genes on the Drosophila X chromosome, Genes & Dev., 17(11), 1334-1339.

• Oh H, Bai X, Park Y, Bone JR, and Kuroda MI. (2004) Targeting dosage compensation to the X chromosome of Drosophila males. Cold spring Harbor Symposium on Quantitative Biology Vol. 69. Epigenetics. (In press)